EPCAM

Epithelial cell adhesion molecule. UniProt annotates extracellular residues 24–265.

Published binding interface

Structure 6I07 records EPCAM in contact with EPCAM-binding scFv. Select the contact residues to inspect this interface.

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RNA expression by cell type

Percentage of cells with detected EPCAM RNA in the public colon adenocarcinoma cell groups.

Tumor cells61.6%

Macrophages (group B)17.5%

Endothelial cells16.4%

Regulatory T cells14.3%

Fibroblasts13.2%

CD8 T cells13.1%

Dendritic cells11.1%

Natural killer cells10.7%

B cells10.6%

Macrophages (group A)10.5%

Granulocytes10.1%

CD4 T cells9.8%

Adipocytes6.4%

RNA detection · Cancer Surfaceome Atlas, Table S6, row 28. Source study · Measurements (JSON)

Inspect the molecular structure

Published reference structure

EPCAM with an antibody fragment

Deposited structure 6I07. EPCAM ectodomain bound by an scFv.

PDB entry: 6I07
Target ID: T-ENSG00000119888

EPCAM-binding scFv · chains A, B (ribbon)
EPCAM ectodomain · chains C, D (ribbon)

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Sequence & binding sites

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Sequence annotations and source records

Canonical sequence and topology

Target ID: T-ENSG00000119888
UniProt accession: P16422
Sequence state: available
Canonical sequence: Yes
Length in amino acids: 314
Sequence SHA-256: e5d527336d82e9309b5ea19a32a85ad410a303db94b245801462e791abd1623c

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Canonical amino-acid sequence

MAPPQVLAFGLLLAAATATFAAAQEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDESGLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEKDVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLKAGVIAVIVVVVIAVVAGIVVLVISRKKRMAKYEKAEIKEMGEMHRELNA

Sequence positions 1–314

Gray shows the full canonical sequence. Colored spans mark the listed intervals.

Signal peptide

1–23

Membrane-spanning segments

266–288 (Helical)

Source-annotated domains

24–265 (Extracellular); 289–314 (Cytoplasmic)

Source-annotated extracellular intervals

24–265

UniProt annotates the following extracellular intervals.

One-based positions include both interval endpoints.
Start	End	Length	Sequence and source
24	265	242	Sequence and source Basis reviewed-uniprot-topological-domain Evidence class source-annotated-topology Sequence SHA-256 f1491c6591e71e270b694e92fe9cd9503488b559d18c7cd98458d699bbce43de Amino-acid sequence for this intervalQEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDESGLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEKDVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLK

Sequence and topology sources

Source role: canonical-sequence
Source: alphafold-db-canonical-uniprot-field
Extracted field: uniprotSequence
Source row accession: Not recorded

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Open sequence source

Source role: reviewed-topology
Source: UniProt reviewed human surface-field snapshot
Extracted field: Not recorded
Source row accession: P16422

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subcellular_location: SUBCELLULAR LOCATION: Lateral cell membrane {ECO:0000269|PubMed:15195135, ECO:0000269|PubMed:16054130, ECO:0000269|PubMed:19785009}; Single-pass type I membrane protein {ECO:0000269|PubMed:16054130}. Cell junction, tight junction {ECO:0000269|PubMed:16054130}. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction. {ECO:0000269|PubMed:16054130}.
transmembrane: TRANSMEM 266..288; /note="Helical"; /evidence="ECO:0000255"
topological_domain: TOPO_DOM 24..265; /note="Extracellular"; /evidence="ECO:0000255"; TOPO_DOM 289..314; /note="Cytoplasmic"; /evidence="ECO:0000255"
signal_peptide: SIGNAL 1..23; /evidence="ECO:0000255"
lipidation: Not recorded

Download all canonical target sequences (FASTA)

Protein identity and location

Protein: Epithelial cell adhesion molecule
Protein location: Transmembrane protein
Canonical sequence: P16422 · 314 amino acids
Extracellular region: UniProt annotates extracellular residues 24–265.

More structures and generated models

Structure snapshots

Expression in normal tissues

RNA specificity: Tissue enhanced
RNA distribution: Detected in many
RNA tissue-specific nTPM: intestine: 756.2
Protein specificity: Tissue enhanced
Protein distribution: Detected in many
Immunohistochemistry reliability: Enhanced
Immunofluorescence reliability: Supported
Protein tissue-specific intensity: intestine: 20,770,505.4
Tissue cell-type enrichment: Breast - Breast glandular cells, Colon - Colon enterocytes, Liver - Cholangiocytes, Lung - Alveolar cells type 2, Minor Salivary Gland - Minor salivary glandular cells, Skin - Eccrine sweat gland cells, Testis - Spermatogonia, Thyroid gland - Thyroid glandular cells
Subcellular location: Plasma membrane

Human Protein Atlas: EPCAM

Structures

Structures records linked to EPCAM.
Name	Stable ID	Action	Format	Status	Claim
6I07	S-RCSB-6I07	Not recorded	Not recorded	not recorded	verified deposited experimental structure

Sources and downloads

UniProt protein record Target measurements (JSON)Sequence annotations (JSON)Target sequences (FASTA)

EPCAM · ENSG00000119888 · P16422