Surface AtlasMucinous colon cancer

Protein-binder design

Explore protein designs for integrin αvβ6 and CEACAM5. Compare their predicted complexes with published binding interfaces and inspect the recorded design outcomes.

Choose a target

Integrin αvβ6 designs

Inspect generated proteins, their target complexes, and amino-acid sequences.

Explore integrin designs →

CEACAM5 designs

Compare proteins generated by three methods and open their saved 3D models.

Explore CEACAM5 designs →

Looking for small molecules? View published binding structures and screening poses →

What each design study produced

Integrin αvβ6Published site · 8TCG
  1. Generate proteins16 backbone and sequence designs.
  2. Predict complexes14 predictions scored; 2 could not be scored.
  3. Check the interface0 met the reference-site geometry criteria.
CEACAM5Original generator comparison · 8BW0
  1. Compare generators3 methods returned 9 candidates.
  2. Check local geometry4 candidates passed these checks.
  3. Common independent screenDid not run: FreeBindCraft returned no candidate.

Proteins generated for integrin αvβ6

This campaign generated 16 protein binder designs for integrin αvβ6. Each design has an amino-acid sequence and a three-dimensional model. Open a generated binder, inspect its position against the target, or download its sequence.

The 14 scored complexes failed the binding-site geometry checks. Two further predictions could not be scored.

Prepared target

Target-only coordinates derived from deposited structure 8TCG. The design input retains alpha-v chain A and beta-6 chain B.
Integrin αvβ6 · prepared using PDB 8TCG.

Generated protein binder

ITGB6 target (chain C); binder rfd3-8tcg-01-mpnn-01 (chain A). Source status: scored. Predicted complex selected. Ranking: unranked.
Integrin binder · design 1A with its integrin target.

Candidate scores and reference controls

Candidate measurements

Campaign target: ITGB6.

Imported candidate records
16
Promoted by recorded decision
0
Control records
2
Scored candidate predictions
14

14 candidate predictions scored; 2 candidate predictions failed local scoring; 2 reference-control predictions scored.

Campaign status

Campaign status

Campaign
8tcg-itgav-itgb6-geometry-recovery
Target
T-ENSG00000115221
Execution
partial
Control calibration
passed
Validated backbones
8
Designed candidates
16
Cofold observations
18
Recorded stop reason
No generated candidate passed the frozen reference-site geometry gate; finalist rescore was not launched.

Design sequences and model results

Generated candidates with recorded cofold metrics, promotion decisions, and hash-verified files.
Generated proteinTarget and purposeProtein sequenceModel measurementsModel checkSource files
Integrin binder · design 1AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsKLPDLGILRRLVRSVARLAVKLGLEELAKKLLEAFANKDYDEAIKLCEEIKKLAPDSESIHKAIEALKKIKA
Download sequence
ipSAE minimum, median
0.394
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 1BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsELPDDGILRRLVEACADAAVDLGLEDTAAALRAAFAAKDYDTAIALLEEIKAKDPDNEAITKAITALKAKKA
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 2AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsSDELKKEADSEIARADYYEAKGKKGNSAYYYDQAAGSYKKAAELYKKLGKEEEAKECNAKAAACQAKADAIR
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 2BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsAEELKKEADQLAALADYHKARGDKANSAYHYDQAAGAYNKAAELYKKLGNEEKAAEYKAKADAYDAKAKAIR
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 3AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsMTLEESVAELLAKKAKRPGNRNTARQLTLIAIKLMEEGKLEEALELAEEALKADPNNEAARKVLEEVKKKIA
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 3BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsMSLEESLARLRAKAAKRPSNRNVARQLALAAIYLAKQGRLEEALAVARAALAADPNNEAARRVLAEVEAKLA
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 4AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsPSGSQVRGLAGLVARRVQEGTPAAELTALVQASLGLSAAAAAAAVARIAEAAAKRDGVAATRALRDAVRAQR
Download sequence
ipSAE minimum, median
0.011
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 4BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsPSGSQVRGLAGKIARLIQEGVPREELVARLQASLGLSEAAARAAVDAIAAAAEKLDGVAAYHALVDAVRAER
Download sequence
ipSAE minimum, median
Not recorded
Target-contact recall, median
Not recorded
Scored observations
0
Failed observations
1
Complex could not be scored
8 verified files
Integrin binder · design 5AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsDPLSGAKAKAATLGVLAAVSEVTKETGVEVSEELKSELIKEAKAALEGKAVTAKEAFELAYEIAKKRLLEKV
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 5BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsAPLSGAKRKAATLGVLAAVSEVEKETGVKVSEETRSELIKKAKEALKGKNVTNKEAFEIAYEIAYKELKEKV
Download sequence
ipSAE minimum, median
0.391
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 6AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsEKKEKAKELIEEAVRLAKEGKVKEALEKVKEATELADNNPEVALLAYTAVRDAAAGLTDDAERAKKKAVELI
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 6BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsEKKEKAIKLVEEAVELAARGEVKKARELVKKATELANNNPEFALLADSAVRDAAAGDFDDAARAAKKAAELL
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 7AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsAEEKIKADPNNSNAWYTLGLEYYAKGNYKEAAEYFEKALAIDPRYVKAHTMLSRVYKKLGNKEKADEIAEKR
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 7BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsYQAKIAADPNNSRAWYALGKEYFAKGDYKNAAAAFEKAIAIDPTFVQAHTYLSRVYNKLGDKAKADAVAEKR
Download sequence
ipSAE minimum, median
Not recorded
Target-contact recall, median
Not recorded
Scored observations
0
Failed observations
1
Complex could not be scored
8 verified files
Integrin binder · design 8AIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsKIEEAIKAAEEGNLEKAIKLAKEAAKLARSNMETVKYLGSAIKAAKEGNLEKAIESLKKALEIAKKRGLEEA
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files
Integrin binder · design 8BIntegrin αvβ6 (ITGB6)Intended effect: block target activityT-ENSG00000115221
View 72 amino acidsLIQKAIDAAKAGDLDKAIALLKEAAKKARGNQETVKWIGAAIEAAKAGDLDGAIAAAEKALENAKKAGDTEA
Download sequence
ipSAE minimum, median
0
Target-contact recall, median
0
Scored observations
1
Failed observations
0
Binding-site geometry checks failed
11 verified files

Control records

Recorded campaign target: ITGB6.

Control records retain their target, sequence, and recorded measurements.
ControlClassTargetSequenceRecorded measurementsFilesClaim
control-positive-8tcg-chain-ccontrol-positive-8tcg-chain-cdeposited-positiveNot recorded
72 aa sequenceCVVRFVFRGDLAELMLRAVKDHLKKEGPHWNITSTNNGKELVVRGIHESDAKRIAKWVEKRFPRVHTETQCD
ipSAE minimum, median
0.579
Target-contact recall, median
0.955
Scored observations
1
Failed observations
0
6 verified files
computational-design-hypothesis
control-negative-composition-shufflecontrol-negative-composition-shuffletarget-matched-negativeNot recorded
72 aa sequenceVLWGILDSHVHRDLFFTVTPLKGDERCAVWKRHEKRNAVQGEFHKVVGERITMKSNEKRAKDCRPTIVNAEL
ipSAE minimum, median
0
Target-contact recall, median
0.091
Scored observations
1
Failed observations
0
6 verified files
computational-design-hypothesis

Proteins generated for CEACAM5

Three design methods generated proteins for the published tusamitamab binding site on CEACAM5. Each design has a saved sequence and a model with the target.

The design pose shows the generator’s proposed placement. Four designs also have a separate complex prediction. Two passed its geometry checks; the reference controls failed, so candidate selection stopped. Read the prediction results and controls →

BoltzGen

CEACAM5 in green with the generated BoltzGen design 03 in blue.
BoltzGen design 03 · green: CEACAM5; blue: generated protein

Design 0164 amino acids

View sequence and downloadMTWKVTFSDGRTGTTEGKPEVGKVLTVTYDDGSVSKVEVVEVVDDNTALVRNLETGEEADATAVDownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/boltzgen/smoke/boltzgen-000

Design 0264 amino acids

View sequence and downloadGTAYATANLTKEEDVVGDIKATVVGTLPGGVKIVKATNLKSGAEVLHDVPGSKETATLAVPSSSDownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/boltzgen/scale/boltzgen-000

Design 0364 amino acids

Predicted-complex geometry checks passed.

View sequence and downloadMEVTVTITKTSYLTDENIKSIIRSALLKAGVSEEEAEKLAEEALAKIKAAKPKVGETVTVTLKVDownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/boltzgen/scale/boltzgen-001

Proteina–Complexa

CEACAM5 in green with the generated Proteina–Complexa design 03 in blue.
Proteina–Complexa design 03 · green: CEACAM5; blue: generated protein

Design 0164 amino acids

View sequence and downloadQAFREFDKDGNGYISTKELGTVMRSLGQNPTEAELQDMINEQDADGDGTISFEEFSTMMRSLGMDownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/proteina-complexa/smoke/proteina-complexa-000

Design 0264 amino acids

Predicted-complex geometry checks failed.

View sequence and downloadSKLKQLRVEHLERTLLEGVLENGQVTFPTLTAEDYELLRTLPRDKQELLAQRFNVSVESFCPAADownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/proteina-complexa/scale/proteina-complexa-000

Design 0364 amino acids

Predicted-complex geometry checks failed.

View sequence and downloadNIYLNLALLYEKLGNKEGAAEAYLQAAQIFYQLGNTDLAIKAAEQANKIYEELGDTSKILNLTSDownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/proteina-complexa/scale/proteina-complexa-001

PXDesign + ProteinMPNN

CEACAM5 in green with the generated PXDesign + ProteinMPNN design 03 in blue.
PXDesign + ProteinMPNN design 03 · green: CEACAM5; blue: generated protein

Design 0164 amino acids

View sequence and downloadSQIDELQAQLQQLQAQLQALVAEAQAAAAAGDKAKLLALLAQTDQLQQQLNQLNQQLVQAKLAADownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/pxdesign-proteinmpnn/smoke/pxdesign-000-proteinmpnn-00

Design 0264 amino acids

View sequence and downloadSAAKQAIADLVQQAAALVQAHNDQVKAMIESGTYDPEQLKQLQEQLQAQLAALQAQIDAAKAAADownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/pxdesign-proteinmpnn/scale/pxdesign-000-proteinmpnn-00

Design 0364 amino acids

Predicted-complex geometry checks passed.

View sequence and downloadTPEEEELRKLTEESSKFQKEMEEKAREAVENNDMEKYKKINEETKKKLEEMNKKNAEIRAKLAADownload sequence (FASTA)Source ID: ceacam5-8bw0-multigenerator/pxdesign-proteinmpnn/scale/pxdesign-001-proteinmpnn-00

Original design comparison

Three methods generated nine CEACAM5 binders; four passed local geometry checks. The common independent screen did not run because FreeBindCraft returned no candidate.

Generator outputs and local design-pose checks
GeneratorCandidates returnedLocal checks
BoltzGen31 pass · 2 fail
Proteina–Complexa32 pass · 1 fail
PXDesign + ProteinMPNN31 pass · 2 fail
FreeBindCraftExecution failed; no candidate returned00 pass · 0 fail

PXDesign + ProteinMPNN: The pass count applies the campaign's frozen 2.0 Å heavy-atom clash cutoff; the arm-native summary also reports a separate 2.2 Å warning metric.

Target, local criteria, and source hash
Target
CEACAM5
Target construct
8BW0 chain C residues 500-676 normalized to campaign A1-A177
Target SHA-256
3420ce3de4f72f60bdbedba11698551167d21577a0b2cc1a76c6f3a2aa148764
Site ID
8bw0-tusamitamab-a3-b3-face
Site residues
17
Binder length
64
Maximum target alignment RMSD (Å)
2.0
Maximum heavy-atom clash pairs
2
Minimum heavy-atom contact pairs
20
Minimum full-footprint residues
6
Minimum heavy-chain contact-footprint residues
4
Evidence class
computational-candidate-hypothesis
Original outcome SHA-256
2df0256aaaee71c25d6e10a23de42d6225ecfe63bf12dd0bd2b6ef8f26cfd889
Predictions with controls

Predictions with controls

A separate study predicted four candidates from the generator comparison with positive and shuffled controls. The controls failed the recorded criteria, which stopped candidate selection.

Reference controls

The same prediction and scoring steps were applied to both controls.
MeasurementPositive controlShuffled control
Interface score (ipSAE-min)0.2164340.0
Reference-site residues contacted913
Target alignment RMSD (Å)2.45171.9697
Control identifiers
Positive control
control-positive-8bw0-tusamitamab-vh
Shuffled control
control-negative-8bw0-vh-composition-shuffle

Recorded failure reasons

  • Positive-control ipSAE below the required minimum
  • Required footprint-contact margin not met
Required positive-control ipSAE-min
0.25
Required positive-minus-negative footprint margin (residues)
4

Candidates that passed geometry before the control stop

  • ceacam5-cp3-boltzgen-03
  • ceacam5-cp3-pxdesign-proteinmpnn-03

The failed control gate prevented these candidates from advancing.

Arms without a passing candidate: proteina-complexa.

Scoring recovery and control outcome
Executed at
2026-09-04
Recorded state
executed-stop-gate-control-failure
Local recovery
Local remeasurement
Local scoring failure
The structure predictions completed. Initial interface scoring failed because the target-site definition omitted the required atom selection. Local remeasurement recovered the scores.
Recovery basis
The frozen 8BW0 site definition and deposited-interface geometry both use non-hydrogen atom contacts; no site, cutoff, chain, model, seed, or gate changed.
Evidence class
computational-candidate-hypothesis

Study outcomes

Study protocol

Download the campaign summary

Paired antibody-domain controls

Paired-variable-domain control diagnostic

Stopped after 1 of 6 predictions

The CEACAM5 + VH + VL positive-control prediction completed. A local scoring incompatibility stopped the run before the negative-control or repeat predictions were issued.

VH and VL are the variable domains of the antibody’s heavy and light chains.

  • The construct is paired VH+VL/Fv-like context: A=CEACAM5 177 aa, B=VH 117 aa, C=VL 104 aa.
  • One structure prediction completed, with four verified structure and confidence files.
  • A separate local analysis treated antibody chains B and C as one partner. It returned a group interface score (ipSAE-min) of 0.0, contacts with 12 of 17 reference-site residues, and a target-alignment RMSD of 2.1726 Å.
  • The full two-control, three-seed panel is incomplete and was not qualified.
  • No generated candidates were included or promoted.
Source records and measurement details
Campaign ID
ceacam5-8bw0-paired-fv-context-diagnostic
Executed on
2026-09-04
Execution
{"authorized_predictions":6,"completed_control":"positive","completed_predictions":1,"completed_seed":20260931,"issued_predictions":1,"unissued_predictions":5}
Paired-group measurement
{"full_footprint_contact_count":12,"full_footprint_residue_count":17,"group_ipsae_min":0.0,"interpretation":"One positive observation cannot evaluate a six-observation paired-control panel. It does not support an inference about paired versus single-variable-domain context.","state":"measured-single-positive-only","target_alignment_rmsd_angstrom":2.1726}
Panel qualification
{"failed_gate_assessment":"not-run","generated_candidates":0,"promotions":0,"qualified":false,"state":"not-evaluable-incomplete-panel"}
Relationship to completed checkpoint
none; the existing three-arm checkpoint was not modified or reinterpreted
Original-source hashes
{"role":"report-handoff","sha256":"72f9d08fc68ec10f46440638ccffdb5b84e44e7b42504375496171e05fef02c2","verification":"hash computed from selected source bytes"}, {"role":"diagnostic-outcome","sha256":"c0cff32084c2f5a030bdda3dfc89afe9b8fb7dad1a0f96816bca1643b1f0f860","verification":"hash computed from selected source bytes"}, {"role":"diagnostic-plan","sha256":"be3b63444df4f9b6f1967c087cd3f0cff2a6baa6a13678e9ed4faeb2a0ee28c5","verification":"hash declared by outcome; artifact not read for this projection"}, {"role":"complex-pdb","sha256":"3f188d647fadc176a3445151ec0e0a7821bf2f6b92dd92546ba2e05275cf12e4","verification":"hash declared by outcome; artifact not read for this projection"}, {"role":"pae-json","sha256":"98b8517022d6ecc5e9e54660069dffa46ba430da9d55f14ab099eebc57e72a04","verification":"hash declared by outcome; artifact not read for this projection"}, {"role":"paired-group-measurement","sha256":"77b9b238c69ec8d6fe6da7b0bfb8f5cd46105b09f971f6354f0ab5983228b1c6","verification":"hash declared by outcome; artifact not read for this projection"}
Source records
Source hashes identify the original input records.

Download reader diagnostic

Replacement-generator study

Replacement generator results

Recorded requests and candidate outcomes.
Candidates passing geometry checks0
Independent validation requests0
Promoted candidates0

ESMFold2: unconditioned generation. This arm did not condition generation on the target site.

  • Genie3 stopped after its initial test: target-coordinate recovery RMSD was 0.0224 Å against a 0.001 Å maximum.
  • ProteinMPNN and Genie3 scale were not called.
  • ESMFold2-design remained a separate unconditioned exploratory arm and was not compared as site-conditioned.
  • All three ESMFold2 candidates failed unchanged full-footprint and VH-subsite gates, so no independent predictor calls were made.
  • No candidate was promoted.
Result records and hashes
Campaign ID
ceacam5-8bw0-replacement-generators-20260904
Recorded status
complete-no-promotions
Results
{"esmfold2_unconditioned_64aa_candidates":3,"genie3_ca_traces":1,"genie3_gate_passers":0,"geometry_passers":0,"independent_validation_eligible":0,"promotions":0}
Claim ceiling
computational-screening-hypothesis
Original outcome SHA-256
5356c67f95caf227d74f35e113e79ce7b174f61794ffd8b19e9cb18b368e983e
Declared artifact hashes
{"campaign_plan":"9483f45c0dd767d30078508830fe006c88d87cd5d76e1f931ad89be5950a34e1","cleanup_snapshot":"322bbb6d2e1d6804b845a2a830699714cea319e189d0a7c820d8a055ec606982","esmfold2_geometry_metrics":"07b86c028edd35bac9caea65b6bcfcf013dfdca698d25882f80757093a25e1ef","esmfold2_normalized_manifest":"c6cfc85ff4d4cafcfca8aeab8b3064f882cf6cbda91940b2921082292fe88652","genie3_smoke_audit":"daa7114a1c5033bab1148c64203c500b7d7497dba8377ae21ac9121df4fee1cf","preflight":"cdf994e5ea28d0eee2e3c16a1fc12c88d202a9a13484c1d151db8449c56612d3"}
Declared final-summary SHA-256
ace5d509f41dfb566aaa49240fe231eddafa1b8d80fad6fe58e95cfd89acd78f
Artifact verification
Hashes declared by the outcome; referenced artifacts were not read for this projection.
Source records
The source hash identifies the original outcome record.
Candidate sequences and downloadable records

Candidate and control sequences

Download the original FASTA files or copy an amino acid sequence.

CEACAM5 checkpoint candidates and controls

BoltzGen 03

Designed candidate · chain B · 64 amino acids

ceacam5-cp3-boltzgen-03

Show sequence

MEVTVTITKTSYLTDENIKSIIRSALLKAGVSEEEAEKLAEEALAKIKAAKPKVGETVTVTLKV

Proteina–Complexa 02

Designed candidate · chain B · 64 amino acids

ceacam5-cp3-proteina-complexa-02

Show sequence

SKLKQLRVEHLERTLLEGVLENGQVTFPTLTAEDYELLRTLPRDKQELLAQRFNVSVESFCPAA

Proteina–Complexa 03

Designed candidate · chain B · 64 amino acids

ceacam5-cp3-proteina-complexa-03

Show sequence

NIYLNLALLYEKLGNKEGAAEAYLQAAQIFYQLGNTDLAIKAAEQANKIYEELGDTSKILNLTS

PXDesign–ProteinMPNN 03

Designed candidate · chain B · 64 amino acids

ceacam5-cp3-pxdesign-proteinmpnn-03

Show sequence

TPEEEELRKLTEESSKFQKEMEEKAREAVENNDMEKYKKINEETKKKLEEMNKKNAEIRAKLAA

Tusamitamab heavy-chain variable domain (VH)

Positive control · chain B · 117 amino acids

control-positive-8bw0-tusamitamab-vh

Show sequence

VQLQESGPGLVKPGGSLSLSCAASGFVFSSYDMSWVRQTPERGLEWVAYISSGGGITYAPSTVKGRFTVSRDNAKNTLYLQMNSLTSEDTAVYYCAAHYFGSSGPFAYWGQGTLVTV

Shuffled tusamitamab VH

Negative control · chain B · 117 amino acids

control-negative-8bw0-vh-composition-shuffle

Show sequence

DEYLPGYGNRVVGSLSSQCESGTWVHVGVRLREYTLYLPTRYSGLSMYSYCANASTGGASKLFFQGITAPTDIVTNGKMAAQAGTGAPGKSEPLVSSFSAVSWYQLVFTWDGQVSFS

Paired control: heavy-chain variable domain (VH)

Paired antibody control · chain B · 117 amino acids

control-positive-8bw0-paired-vh-vl

Show sequence

VQLQESGPGLVKPGGSLSLSCAASGFVFSSYDMSWVRQTPERGLEWVAYISSGGGITYAPSTVKGRFTVSRDNAKNTLYLQMNSLTSEDTAVYYCAAHYFGSSGPFAYWGQGTLVTV

Paired control: light-chain variable domain (VL)

Paired antibody control · chain C · 104 amino acids

control-positive-8bw0-paired-vh-vl:vl

Show sequence

DIQMTQSPASLSASVGDRVTITCRASENIFSYLAWYQQKPGKSPKLLVYNTRTLAEGVPSRFSGSGSGTDFSLTISSLQPEDFATYYCQHHYGTPFTFGSGTKL

Design data

The exports preserve imported sequences, measurements, model versions, decisions, and source payloads.

Downloads:Binders JSONBinders CSVBinder controls JSONBinder campaigns JSON

Candidate sequences and design poses

Recover each candidate’s amino-acid sequence and generated design pose. Candidate IDs include the campaign, generator arm and run stage.

Download candidate file index

BoltzGen

ceacam5-8bw0-multigenerator

boltzgen-000 · smoke

Campaign tool: BoltzGen.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose. CEACAM5 target (chain A); binder boltzgen-000 (chain B). Source status: generated. Screening state: not run; four-arm join incomplete. Ranking: unranked.
Generated design pose. CEACAM5 target (chain A); binder boltzgen-000 (chain B). Source status: generated. Screening state: not run; four-arm join incomplete. Ranking: unranked.

· Download PNG · Download render settings

Download FASTA · Download generated design pose (PDB)

Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/boltzgen/smoke/boltzgen-000
Sequence SHA-256
28152b2f7418717c770fce2bb3522edb44b1e0b4b1aaafeb5e22cf802452ac57
Original manifest SHA-256
25f7d4ffc507df18384e92a53554ae5d654348355740e2d9f3b5ecd7b412b5f5
Original pose SHA-256
34c405561f55e41909a0f13fb04fa9abd6c5f0a25df7fa64281bf735bcf9da3e
Packaged pose SHA-256
34c405561f55e41909a0f13fb04fa9abd6c5f0a25df7fa64281bf735bcf9da3e
Observed protein-chain lengths
{"A":177,"B":64}

boltzgen-000 · scale

Campaign tool: BoltzGen.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose. CEACAM5 target (chain A); binder boltzgen-000 (chain B). Source status: generated. Screening state: not run; four-arm join incomplete. Ranking: unranked.
Generated design pose. CEACAM5 target (chain A); binder boltzgen-000 (chain B). Source status: generated. Screening state: not run; four-arm join incomplete. Ranking: unranked.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/boltzgen/scale/boltzgen-000
Sequence SHA-256
f3e48bd663bfecfb774556bbd3c8537fba17265d4869eaaf422fda1eb3b99455
Original manifest SHA-256
ce59cdb6e4c27c71b0a453a23733d874adef5f845376405878d2cd3f631f7af4
Original pose SHA-256
e61bd7d95f6c3f5e63bd0287696176fd6e146860f9624a93ee2ffbdc32ef7b9b
Packaged pose SHA-256
e61bd7d95f6c3f5e63bd0287696176fd6e146860f9624a93ee2ffbdc32ef7b9b
Observed protein-chain lengths
{"A":177,"B":64}

boltzgen-001 · scale

Campaign tool: BoltzGen.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.
Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/boltzgen/scale/boltzgen-001
Sequence SHA-256
ccc912f3587e2527d082c7e39b439feda882f3a71a006545ee7561754977fcd2
Original manifest SHA-256
ce59cdb6e4c27c71b0a453a23733d874adef5f845376405878d2cd3f631f7af4
Original pose SHA-256
2c2ce0876e1495fa8014ff72e2d33071b1379585d9af004d9d4ae5d4326fb07f
Packaged pose SHA-256
2c2ce0876e1495fa8014ff72e2d33071b1379585d9af004d9d4ae5d4326fb07f
Observed protein-chain lengths
{"A":177,"B":64}

Proteina–Complexa

ceacam5-8bw0-multigenerator

proteina-complexa-000 · smoke

Campaign tool: Proteina–Complexa.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose. CEACAM5 target (chain A); binder proteina-complexa-000 (chain B). Source status: generated. Screening state: not run; four-arm join incomplete. Ranking: unranked.
Generated design pose. CEACAM5 target (chain A); binder proteina-complexa-000 (chain B). Source status: generated. Screening state: not run; four-arm join incomplete. Ranking: unranked.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/proteina-complexa/smoke/proteina-complexa-000
Sequence SHA-256
f3fcb1023fa52085b79f633d631390733c868ee809fa84a0765cc5718691e443
Original manifest SHA-256
e8b3451955d98b9ac7f8f659acf3bea931b8874b7db5fa6cb029e9384e83e15c
Original pose SHA-256
a7b368ee080db8d30c21df05180ce704cbe2585f88f8071641b9742852e4ff99
Packaged pose SHA-256
a7b368ee080db8d30c21df05180ce704cbe2585f88f8071641b9742852e4ff99
Observed protein-chain lengths
{"A":177,"B":64}

proteina-complexa-000 · scale

Campaign tool: Proteina–Complexa.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.
Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/proteina-complexa/scale/proteina-complexa-000
Sequence SHA-256
238c73b712b8664a3722299b5ed7cc094548c5723ff37792d7e5b5d1d0c42414
Original manifest SHA-256
7b1a74b161ad50d73e83e09fc919f49c97d417349c6d174825f67f261e1a38fa
Original pose SHA-256
cd6f93802c466ab7e0c2f79f58c1563e3938edf92b661770d5de9e10d5974a4d
Packaged pose SHA-256
cd6f93802c466ab7e0c2f79f58c1563e3938edf92b661770d5de9e10d5974a4d
Observed protein-chain lengths
{"A":177,"B":64}

proteina-complexa-001 · scale

Campaign tool: Proteina–Complexa.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.
Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/proteina-complexa/scale/proteina-complexa-001
Sequence SHA-256
493fcdfa980f4f969d0729a1fc59983ebfc076b1b3100ef4610e6383034dcff0
Original manifest SHA-256
7b1a74b161ad50d73e83e09fc919f49c97d417349c6d174825f67f261e1a38fa
Original pose SHA-256
6fe046449c149dab304990ea2e7bd7074e076ffc4552e78a13c178b7423516d1
Packaged pose SHA-256
6fe046449c149dab304990ea2e7bd7074e076ffc4552e78a13c178b7423516d1
Observed protein-chain lengths
{"A":177,"B":64}

PXDesign + ProteinMPNN

ceacam5-8bw0-multigenerator

pxdesign-000-proteinmpnn-00 · smoke

Campaign tool: PXDesign + ProteinMPNN.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose. CEACAM5 target (chain A); binder pxdesign-000-proteinmpnn-00 (chain B). Source status: sequence-designed. Screening state: not run; four-arm join incomplete. Ranking: unranked.
Generated design pose. CEACAM5 target (chain A); binder pxdesign-000-proteinmpnn-00 (chain B). Source status: sequence-designed. Screening state: not run; four-arm join incomplete. Ranking: unranked.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/pxdesign-proteinmpnn/smoke/pxdesign-000-proteinmpnn-00
Sequence SHA-256
b35b51b58394aca742cc52fbc6b007dc46becd6b54a1ba309638b32035d7c890
Original manifest SHA-256
20a58ba531e6dd334ae3d8fa10ecbfc86f73cd4c7eb074f923dae39e25857c12
Original pose SHA-256
f48fdd7750b42edb7de1fbdb17c73ed1e73f2ac9af865d2168e308a847eaeea2
Packaged pose SHA-256
b37beb34cc69cee7a56382116e29badb68cb6250d2bc2f9e78f5c40c0a5e7863
Observed protein-chain lengths
{"A":177,"B":64}

pxdesign-000-proteinmpnn-00 · scale

Campaign tool: PXDesign + ProteinMPNN.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose. CEACAM5 target (chain A); binder pxdesign-000-proteinmpnn-00 (chain B). Source status: sequence-designed. Screening state: not run; four-arm join incomplete. Ranking: unranked.
Generated design pose. CEACAM5 target (chain A); binder pxdesign-000-proteinmpnn-00 (chain B). Source status: sequence-designed. Screening state: not run; four-arm join incomplete. Ranking: unranked.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/pxdesign-proteinmpnn/scale/pxdesign-000-proteinmpnn-00
Sequence SHA-256
b27052a7063360ba8a2da4b62d37e8a782f3c3826b7065dff1f3f14fdff2e119
Original manifest SHA-256
b332a91243e635cc40f60393259a43a198d3f9b625f74f081d0ae862da6d42c6
Original pose SHA-256
35d27a471df43f69e27c548c4a266e11c3dd513475dc348c2d5454487a1d3289
Packaged pose SHA-256
738068413ec8f8c5da845b8c3791eadc800c5d18c50b40350e0e87354da08caf
Observed protein-chain lengths
{"A":177,"B":64}

pxdesign-001-proteinmpnn-00 · scale

Campaign tool: PXDesign + ProteinMPNN.

64 amino acids · Generated design pose · Sequence matches chain B

Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.
Generated design pose from the original four-arm campaign. This candidate was later screened in the three-arm checkpoint; the checkpoint failed its control check and no candidate was promoted.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-multigenerator/pxdesign-proteinmpnn/scale/pxdesign-001-proteinmpnn-00
Sequence SHA-256
6e14cddf2dc1b16095def35925cc6bd00a51174f17e4596200671bd4b0916413
Original manifest SHA-256
b332a91243e635cc40f60393259a43a198d3f9b625f74f081d0ae862da6d42c6
Original pose SHA-256
0e16974db57d524c3d1405f60c33f4d258a827374a64815ed5372dd01b7409dd
Packaged pose SHA-256
4915edf92a1efeadd2b73edf7e0f6ffe49df08835262d848de9d5c0c8f9d6a82
Observed protein-chain lengths
{"A":177,"B":64}

esmfold2-fast-unconditioned

ceacam5-8bw0-replacement-generators-20260904

esmfold2-unconditioned-001 · smoke

64 amino acids · Predicted sequence fold · Sequence matches chain B

Predicted fold of the generated sequence (chain B). The ESMFold2 request included the target sequence but did not specify a binding site. The candidate chain is shown in isolation.
Predicted fold of the generated sequence (chain B). The ESMFold2 request included the target sequence but did not specify a binding site. The candidate chain is shown in isolation.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-replacement-generators-20260904/esmfold2-fast-unconditioned/smoke/esmfold2-unconditioned-001
Sequence SHA-256
32e9f4fba2f34565443d17a74790b7d21d1d2a9807a9ac717e4104d2f58abe7d
Original manifest SHA-256
c6cfc85ff4d4cafcfca8aeab8b3064f882cf6cbda91940b2921082292fe88652
Original pose SHA-256
1122412e01897edc09751158d332caf65bb72b25374acc6f8ed38f6325d24933
Packaged pose SHA-256
1122412e01897edc09751158d332caf65bb72b25374acc6f8ed38f6325d24933
Observed protein-chain lengths
{"A":177,"B":64}

esmfold2-unconditioned-002 · scale

64 amino acids · Predicted sequence fold · Sequence matches chain B

Predicted fold of the generated sequence (chain B). The ESMFold2 request included the target sequence but did not specify a binding site. The candidate chain is shown in isolation.
Predicted fold of the generated sequence (chain B). The ESMFold2 request included the target sequence but did not specify a binding site. The candidate chain is shown in isolation.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-replacement-generators-20260904/esmfold2-fast-unconditioned/scale/esmfold2-unconditioned-002
Sequence SHA-256
15ce01b4c21a6b6745a4dbf4e358d7cf99b4883ce7042419cef3d3e2e99b8a6d
Original manifest SHA-256
c6cfc85ff4d4cafcfca8aeab8b3064f882cf6cbda91940b2921082292fe88652
Original pose SHA-256
80a72746911d491a0051d9ec08febc959098335e772ae15b350ca1ffe849e926
Packaged pose SHA-256
80a72746911d491a0051d9ec08febc959098335e772ae15b350ca1ffe849e926
Observed protein-chain lengths
{"A":177,"B":64}

esmfold2-unconditioned-003 · scale

64 amino acids · Predicted sequence fold · Sequence matches chain B

Predicted fold of the generated sequence (chain B). The ESMFold2 request included the target sequence but did not specify a binding site. The candidate chain is shown in isolation.
Predicted fold of the generated sequence (chain B). The ESMFold2 request included the target sequence but did not specify a binding site. The candidate chain is shown in isolation.

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Exact identifiers and file hashes
Qualified candidate ID
ceacam5-8bw0-replacement-generators-20260904/esmfold2-fast-unconditioned/scale/esmfold2-unconditioned-003
Sequence SHA-256
325ae69ed9e8cd72f1e16ac656d14cbac3b027963a558e6f66e8ec2b7741cc7d
Original manifest SHA-256
c6cfc85ff4d4cafcfca8aeab8b3064f882cf6cbda91940b2921082292fe88652
Original pose SHA-256
b9c391ed3513b318caf321bbdeb2c6f261cd601ced46d7429664540563a9b1f3
Packaged pose SHA-256
b9c391ed3513b318caf321bbdeb2c6f261cd601ced46d7429664540563a9b1f3
Observed protein-chain lengths
{"A":177,"B":64}

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