GJA1

Gap junction alpha-1 protein. UniProt annotates extracellular residues 45–76, 177–207.

Sequence and protein regions

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RNA expression by cell type

Percentage of cells with detected GJA1 RNA in the public colon adenocarcinoma cell groups.

Endothelial cells45.9%

Fibroblasts28.2%

Adipocytes11.2%

Dendritic cells4.6%

Tumor cells1.2%

Macrophages (group B)1.1%

Granulocytes0.7%

Macrophages (group A)0.5%

CD8 T cells0.5%

B cells0.4%

CD4 T cells0.3%

Regulatory T cells0.3%

Natural killer cells0.2%

RNA detection · Cancer Surfaceome Atlas, Table S6, row 94. Source study · Measurements (JSON)

Sequence & binding sites

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Sequence annotations and source records

Canonical sequence and topology

Target ID: T-ENSG00000152661
UniProt accession: P17302
Sequence state: available
Canonical sequence: Yes
Length in amino acids: 382
Sequence SHA-256: 57ac4ca5808e70e179c865f45cbc931a716b9e7786f80621bea69292f074948e

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Canonical amino-acid sequence

MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Sequence positions 1–382

Gray shows the full canonical sequence. Colored spans mark the listed intervals.

Membrane-spanning segments

24–44 (Helical); 77–97 (Helical); 156–176 (Helical); 208–228 (Helical)

Source-annotated domains

2–23 (Cytoplasmic); 45–76 (Extracellular); 98–155 (Cytoplasmic); 177–207 (Extracellular); 229–382 (Cytoplasmic)

Source-annotated extracellular intervals

45–76; 177–207

UniProt annotates the following extracellular intervals.

One-based positions include both interval endpoints.
Start	End	Length	Sequence and source
45	76	32	Sequence and source Basis reviewed-uniprot-topological-domain Evidence class source-annotated-topology Sequence SHA-256 61d8802e45e8d78bf61de4731ddce2c47866be164b9172809ac8ff4abf1de21e Amino-acid sequence for this intervalWGDEQSAFRCNTQQPGCENVCYDKSFPISHVR
177	207	31	Sequence and source Basis reviewed-uniprot-topological-domain Evidence class source-annotated-topology Sequence SHA-256 a372bd1c7e8da214624d0965b42af9d4752e590736a3d9164d344c4f3a0fc247 Amino-acid sequence for this intervalYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKT

Sequence and topology sources

Source role: canonical-sequence
Source: alphafold-db-canonical-uniprot-field
Extracted field: uniprotSequence
Source row accession: Not recorded

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Open sequence source

Source role: reviewed-topology
Source: UniProt reviewed human surface-field snapshot
Extracted field: Not recorded
Source row accession: P17302

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subcellular_location: SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22411987, ECO:0000269|PubMed:25398053}; Multi-pass membrane protein {ECO:0000255}. Cell junction, gap junction {ECO:0000269|PubMed:22411987, ECO:0000269|PubMed:25225338, ECO:0000269|PubMed:25398053, ECO:0000269|PubMed:38375917}. Endoplasmic reticulum {ECO:0000250|UniProtKB:P23242}. Cell junction {ECO:0000269|PubMed:26604139, ECO:0000269|PubMed:34905516}. Note=Localizes at the intercalated disk (ICD) between two adjacent cardiomyocytes and the proper localization at ICD is dependent on TMEM65. {ECO:0000250|UniProtKB:P23242}.
transmembrane: TRANSMEM 24..44; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 77..97; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 156..176; /note="Helical"; /evidence="ECO:0000255"; TRANSMEM 208..228; /note="Helical"; /evidence="ECO:0000255"
topological_domain: TOPO_DOM 2..23; /note="Cytoplasmic"; /evidence="ECO:0000250|UniProtKB:P08050"; TOPO_DOM 45..76; /note="Extracellular"; /evidence="ECO:0000250|UniProtKB:P08050"; TOPO_DOM 98..155; /note="Cytoplasmic"; /evidence="ECO:0000250|UniProtKB:P08050"; TOPO_DOM 177..207; /note="Extracellular"; /evidence="ECO:0000250|UniProtKB:P08050"; TOPO_DOM 229..382; /note="Cytoplasmic"; /evidence="ECO:0000250|UniProtKB:P08050"
signal_peptide: Not recorded
lipidation: Not recorded

Download all canonical target sequences (FASTA)

Protein identity and location

Protein: Gap junction alpha-1 protein
Protein location: Transmembrane protein
Canonical sequence: P17302 · 382 amino acids
Extracellular region: UniProt annotates extracellular residues 45–76, 177–207.

Expression in normal tissues

RNA specificity: Low tissue specificity
RNA distribution: Detected in many
Protein specificity: Tissue enhanced
Protein distribution: Detected in many
Immunohistochemistry reliability: Enhanced
Immunofluorescence reliability: Approved
Protein tissue-specific intensity: heart muscle: 57,590,060.3
Tissue cell-type enrichment: Adipose visceral - Mesothelial cells, Adrenal gland - Adrenal cortex cells, Heart muscle - Cardiomyocytes
Subcellular location: Vesicles, Cell Junctions

Human Protein Atlas: GJA1

Sources and downloads

UniProt protein record Target measurements (JSON)Sequence annotations (JSON)Target sequences (FASTA)

GJA1 · ENSG00000152661 · P17302