MSLN

Mesothelin. An extracellular span at residues 37–597 is derived from the signal peptide and GPI attachment annotation.

Predicted protein structure

A downloaded AlphaFold model connects the MSLN sequence to its predicted three-dimensional structure.

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RNA expression by cell type

Percentage of cells with detected MSLN RNA in the public colon adenocarcinoma cell groups.

Tumor cells21.4%

Fibroblasts4.5%

Macrophages (group B)2.8%

Dendritic cells2.6%

Adipocytes2.3%

Macrophages (group A)1.4%

Endothelial cells1.3%

Regulatory T cells1.0%

Natural killer cells1.0%

CD4 T cells0.5%

CD8 T cells0.5%

Granulocytes0.5%

B cells0.4%

RNA detection · Cancer Surfaceome Atlas, Table S6, row 78. Source study · Measurements (JSON)

Sequence & binding sites

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Sequence annotations and source records

Canonical sequence and topology

Target ID: T-ENSG00000102854
UniProt accession: Q13421
Sequence state: available
Canonical sequence: Yes
Length in amino acids: 630
Sequence SHA-256: 85dadcd8397d68d34972e3278f0fc47b51659537c8c6ba3e62243aee293108b3

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Canonical amino-acid sequence

MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALSGTPCLLGPGPVLTVLALLLASTLA

Sequence positions 1–630

Gray shows the full canonical sequence. Colored spans mark the listed intervals.

Signal peptide

1–36

Lipid attachment sites

598–598 (GPI-anchor amidated serine)

Derived extracellular interval

37–597

The extracellular interval is derived from the signal peptide and GPI attachment site. It is a proposed construct interval.

One-based positions include both interval endpoints.
Start	End	Length	Sequence and source
37	597	561	Sequence and source Basis interval-after-reviewed-signal-before-reviewed-gpi-anchor Evidence class topology-derived-construct-hypothesis Sequence SHA-256 ce81626d0166807d79441701c224844811c962f8598d8b9db957d37c29b225f0 Amino-acid sequence for this intervalLAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVL

Sequence and topology sources

Source role: canonical-sequence
Source: alphafold-db-canonical-uniprot-field
Extracted field: uniprotSequence
Source row accession: Not recorded

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Open sequence source

Source role: reviewed-topology
Source: UniProt reviewed human surface-field snapshot
Extracted field: Not recorded
Source row accession: Q13421

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subcellular_location: SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.; SUBCELLULAR LOCATION: [Megakaryocyte-potentiating factor]: Secreted.; SUBCELLULAR LOCATION: [Isoform 5]: Secreted.
transmembrane: Not recorded
topological_domain: Not recorded
signal_peptide: SIGNAL 1..36; /evidence="ECO:0000269|PubMed:7665620"
lipidation: LIPID 598; /note="GPI-anchor amidated serine"; /evidence="ECO:0000255"

Download all canonical target sequences (FASTA)

Protein identity and location

Protein: Mesothelin
Protein location: GPI-anchored membrane protein
Canonical sequence: Q13421 · 630 amino acids
Extracellular region: An extracellular span at residues 37–597 is derived from the signal peptide and GPI attachment annotation.

Expression in normal tissues

RNA specificity: Tissue enhanced
RNA distribution: Detected in many
RNA tissue-specific nTPM: adipose tissue: 89.2; fallopian tube: 261.5
Protein specificity: Tissue enhanced
Protein distribution: Detected in many
Immunohistochemistry reliability: Enhanced
Immunofluorescence reliability: Supported
Protein tissue-specific intensity: fallopian tube: 4,541,510.9
Tissue cell-type enrichment: Adipose visceral - Mesothelial cells, Pancreas - Ductal cells, Prostate - Urothelial cells, Skin - Eccrine sweat gland cells
Subcellular location: Vesicles, Plasma membrane

Human Protein Atlas: MSLN

Structures

A predicted target model is available.

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Sources and downloads

UniProt protein record Target measurements (JSON)Sequence annotations (JSON)Target sequences (FASTA)

MSLN · ENSG00000102854 · Q13421