CD73 (NT5E)

5'-nucleotidase. An extracellular span at residues 27–548 is derived from the signal peptide and GPI attachment annotation.

Molecule screening

The CD73 campaign screened 24 molecules and repeated five selections. All five passed the recorded docking and pose checks.

View campaign results

RNA expression by cell type

Percentage of cells with detected NT5E RNA in the public colon adenocarcinoma cell groups.

Fibroblasts24.1%

Endothelial cells19.0%

Tumor cells12.6%

Adipocytes6.9%

Macrophages (group B)4.3%

B cells4.0%

Macrophages (group A)1.7%

Dendritic cells1.3%

Granulocytes1.0%

CD4 T cells0.9%

CD8 T cells0.8%

Natural killer cells0.7%

Regulatory T cells0.5%

RNA detection · Cancer Surfaceome Atlas, Table S6, row 177. Source study · Measurements (JSON)

Inspect the molecular structure

Published reference structure

CD73 with mAb19

Deposited structure 7BBJ. CD73 dimer bound by the antagonistic antibody mAb19.

PDB entry: 7BBJ
Target ID: T-ENSG00000135318

CD73 ectodomain dimer · chains A, B (ribbon)
mAb19 heavy chain · chains H, M (ribbon)
mAb19 light chain · chains L, N (ribbon)

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Sequence & binding sites

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Sequence annotations and source records

Canonical sequence and topology

Target ID: T-ENSG00000135318
UniProt accession: P21589
Sequence state: available
Canonical sequence: Yes
Length in amino acids: 574
Sequence SHA-256: 0e3740730dd7a0bb4d2d5eb050d6f2e5ead1cdfa625f09f6a7ab65acc340055f

View and select the full canonical sequence

Canonical amino-acid sequence

MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFSTGSHCHGSFSLIFLSLWAVIFVLYQ

Sequence positions 1–574

Gray shows the full canonical sequence. Colored spans mark the listed intervals.

Signal peptide

1–26

Lipid attachment sites

549–549 (GPI-anchor amidated serine)

Derived extracellular interval

27–548

The extracellular interval is derived from the signal peptide and GPI attachment site. It is a proposed construct interval.

One-based positions include both interval endpoints.
Start	End	Length	Sequence and source
27	548	522	Sequence and source Basis interval-after-reviewed-signal-before-reviewed-gpi-anchor Evidence class topology-derived-construct-hypothesis Sequence SHA-256 aed566eaa2b8fbc1297514fb7abf3562ff84f61d230b782dda9827dcc8474606 Amino-acid sequence for this intervalWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKF

Sequence and topology sources

Source role: canonical-sequence
Source: alphafold-db-canonical-uniprot-field
Extracted field: uniprotSequence
Source row accession: Not recorded

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Open sequence source

Source role: reviewed-topology
Source: UniProt reviewed human surface-field snapshot
Extracted field: Not recorded
Source row accession: P21589

Download source snapshot (gzip)

subcellular_location: SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:2129526, ECO:0000269|PubMed:24887587}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:2129526}.
transmembrane: Not recorded
topological_domain: Not recorded
signal_peptide: SIGNAL 1..26; /evidence="ECO:0000269|PubMed:2173922"
lipidation: LIPID 549; /note="GPI-anchor amidated serine"; /evidence="ECO:0000269|PubMed:2129526"

Download all canonical target sequences (FASTA)

Protein identity and location

Protein: 5'-nucleotidase
Protein location: GPI-anchored membrane protein
Canonical sequence: P21589 · 574 amino acids
Extracellular region: An extracellular span at residues 27–548 is derived from the signal peptide and GPI attachment annotation.

More structures and generated models

Structure snapshots

Published reference structure

CD73 with compound 21

Deposited structure 9R0G. CD73 ectodomain bound by compound 21.

PDB entry: 9R0G
Target ID: T-ENSG00000135318

CD73 ectodomain dimer · chains A, B (ribbon)
Compound 21 · carbon in ochre; oxygen red; nitrogen blue (sticks)

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Expression in normal tissues

RNA specificity: Tissue enhanced
RNA distribution: Detected in many
RNA tissue-specific nTPM: cervix: 105.8
Protein specificity: Tissue enhanced
Protein distribution: Detected in all
Immunohistochemistry reliability: Approved
Immunofluorescence reliability: Supported
Protein tissue-specific intensity: ovary: 65,387,181.7
Tissue cell-type enrichment: Adipose subcutaneous - Adipose progenitor cells, Adipose visceral - Adipose progenitor cells, Breast - Fibroblasts, Heart muscle - Fibroblasts, Lung - Fibroblasts_2, Prostate - Fibroblasts, Skeletal muscle - Fibroblasts
Subcellular location: Plasma membrane

Human Protein Atlas: NT5E

Known molecule interactions

Known Molecule Interactions linked to NT5E.
Molecule	Stable ID	Category	Reference or intention	Claim
Quemliclustat	RF007	Reference ligand	source	source-backed known interaction
CD73 compound 21 (PDB CCD A1JCG)	RF019	Reference ligand	source	source-backed known interaction

Molecule screening results

The CD73 campaign screened 24 molecules and repeated five selections. All five passed the recorded docking and pose checks.

Compare molecules, poses, and repeated runs →

Structures

Structures records linked to NT5E.
Name	Stable ID	Action	Format	Status	Claim
7BBJ	S-RCSB-7BBJ	Not recorded	Not recorded	not recorded	verified deposited experimental structure
9R0G	S-RCSB-9R0G	Not recorded	Not recorded	not recorded	verified deposited experimental structure

Sources and downloads

UniProt protein record Target measurements (JSON)Sequence annotations (JSON)Target sequences (FASTA)

NT5E · ENSG00000135318 · P21589