EGFR

Epidermal growth factor receptor. UniProt annotates extracellular residues 25–645.

Published binding interface

Structure 1YY9 records EGFR in contact with cetuximab Fab. Select the contact residues to inspect this interface.

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RNA expression by cell type

Percentage of cells with detected EGFR RNA in the public colon adenocarcinoma cell groups.

Fibroblasts16.0%

Tumor cells11.0%

Adipocytes6.3%

Dendritic cells1.3%

Endothelial cells0.8%

Macrophages (group B)0.8%

Granulocytes0.5%

Regulatory T cells0.5%

B cells0.5%

Macrophages (group A)0.4%

CD4 T cells0.3%

CD8 T cells0.3%

Natural killer cells0.0%

RNA detection · Cancer Surfaceome Atlas, Table S6, row 5. Source study · Measurements (JSON)

Inspect the molecular structure

Published reference structure

EGFR with cetuximab

Deposited structure 1YY9. EGFR extracellular domain bound by cetuximab Fab.

PDB entry: 1YY9
Target ID: T-ENSG00000146648

EGFR extracellular domain · chain A (ribbon)
cetuximab Fab light chain · chain C (ribbon)
cetuximab Fab heavy chain · chain D (ribbon)

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Sequence & binding sites

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Sequence annotations and source records

Canonical sequence and topology

Target ID: T-ENSG00000146648
UniProt accession: P00533
Sequence state: available
Canonical sequence: Yes
Length in amino acids: 1210
Sequence SHA-256: dbb4583267ad40a7dfd854ee3c44ce4be38e137d0570e3eb76cf1854a74e7246

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Canonical amino-acid sequence

MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA

Sequence positions 1–1210

Gray shows the full canonical sequence. Colored spans mark the listed intervals.

Signal peptide

1–24

Membrane-spanning segments

646–668 (Helical)

Source-annotated domains

25–645 (Extracellular); 669–1210 (Cytoplasmic)

Lipid attachment sites

1049–1049 (S-palmitoyl cysteine); 1146–1146 (S-palmitoyl cysteine)

Source-annotated extracellular intervals

25–645

UniProt annotates the following extracellular intervals.

One-based positions include both interval endpoints.
Start	End	Length	Sequence and source
25	645	621	Sequence and source Basis reviewed-uniprot-topological-domain Evidence class source-annotated-topology Sequence SHA-256 be4a09cf6ffda325749e1dbc3969f690c9e5ff242407d8891ddbf8df0726dd37 Amino-acid sequence for this intervalLEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPS

Sequence and topology sources

Source role: canonical-sequence
Source: alphafold-db-canonical-uniprot-field
Extracted field: uniprotSequence
Source row accession: Not recorded

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Open sequence source

Source role: reviewed-topology
Source: UniProt reviewed human surface-field snapshot
Extracted field: Not recorded
Source row accession: P00533

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subcellular_location: SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17182860, ECO:0000269|PubMed:20462955, ECO:0000269|PubMed:23589287, ECO:0000269|PubMed:26918609, ECO:0000269|PubMed:27153536, ECO:0000269|PubMed:2790960, ECO:0000269|PubMed:35538033}; Single-pass type I membrane protein {ECO:0000269|PubMed:27153536}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:27153536}; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome {ECO:0000269|PubMed:17182860, ECO:0000269|PubMed:27153536}. Endosome membrane. Nucleus {ECO:0000269|PubMed:17115032, ECO:0000269|PubMed:17909029, ECO:0000269|PubMed:20551055, ECO:0000269|PubMed:20674546}. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:17909029, PubMed:20674546). Endocytosed upon activation by ligand (PubMed:17182860, PubMed:17909029, PubMed:27153536, PubMed:2790960). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). {ECO:0000269|PubMed:17182860, ECO:0000269|PubMed:17909029, ECO:0000269|PubMed:20674546, ECO:0000269|PubMed:27153536, ECO:0000269|PubMed:2790960}.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted.
transmembrane: TRANSMEM 646..668; /note="Helical"; /evidence="ECO:0000255"
topological_domain: TOPO_DOM 25..645; /note="Extracellular"; /evidence="ECO:0000255"; TOPO_DOM 669..1210; /note="Cytoplasmic"; /evidence="ECO:0000255"
signal_peptide: SIGNAL 1..24; /evidence="ECO:0000269|PubMed:15340161, ECO:0000269|PubMed:6328312, ECO:0000269|Ref.16"
lipidation: LIPID 1049; /note="S-palmitoyl cysteine"; /evidence="ECO:0000269|PubMed:27153536"; LIPID 1146; /note="S-palmitoyl cysteine"; /evidence="ECO:0000269|PubMed:27153536"

Download all canonical target sequences (FASTA)

Protein identity and location

Protein: Epidermal growth factor receptor
Protein location: Transmembrane protein
Canonical sequence: P00533 · 1,210 amino acids
Extracellular region: UniProt annotates extracellular residues 25–645.

More structures and generated models

Structure snapshots

Expression in normal tissues

RNA specificity: Tissue enhanced
RNA distribution: Detected in many
RNA tissue-specific nTPM: placenta: 61.8
Protein specificity: Low tissue specificity
Protein distribution: Detected in all
Immunohistochemistry reliability: Enhanced
Immunofluorescence reliability: Supported
Tissue cell-type enrichment: Prostate - Prostate basal glandular cells
Subcellular location: Golgi apparatus, Plasma membrane

Human Protein Atlas: EGFR

Structures

Structures records linked to EGFR.
Name	Stable ID	Action	Format	Status	Claim
1YY9	S-RCSB-1YY9	Not recorded	Not recorded	not recorded	verified deposited experimental structure

Sources and downloads

UniProt protein record Target measurements (JSON)Sequence annotations (JSON)Target sequences (FASTA)

EGFR · ENSG00000146648 · P00533