GUCY2C

Guanylyl cyclase C. UniProt annotates extracellular residues 24–430.

Published binding interface

Structure 8GHP records GUCY2C in contact with anti-GUCY2C scFv. Select the contact residues to inspect this interface.

RNA expression in tumor samples

Median GUCY2C RNA expression by tumor type, measured in RSEM units. The study selected these samples using microsatellite-stability score thresholds.

Mucinous tumors37 samples1,896.4

Other tumor types438 samples1,842.6

Compare the sample groups · cBioPortal measurements (JSON)

Inspect the molecular structure

Published reference structure

GUCY2C with an antibody fragment

Deposited structure 8GHP. GUCY2C extracellular domain bound by an anti-GUCY2C scFv.

PDB entry: 8GHP
Target ID: T-ENSG00000070019

GUCY2C extracellular domain · chain A (ribbon)
anti-GUCY2C scFv heavy chain · chain H (ribbon)
anti-GUCY2C scFv light chain · chain L (ribbon)

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Sequence & binding sites

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Sequence annotations and source records

Canonical sequence and topology

Target ID: T-ENSG00000070019
UniProt accession: P25092
Sequence state: available
Canonical sequence: Yes
Length in amino acids: 1073
Sequence SHA-256: 65e0c45e5759303997c3b2d84199fc621c9d3899348c9b6fd17686195676db85

View and select the full canonical sequence

Canonical amino-acid sequence

MKTLLLDLALWSLLFQPGWLSFSSQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQILMIAVFTLTGAVVLLLLVALLMLRKYRKDYELRQKKWSHIPPENIFPLETNETNHVSLKIDDDKRRDTIQRLRQCKYDKKRVILKDLKHNDGNFTEKQKIELNKLLQIDYYNLTKFYGTVKLDTMIFGVIEYCERGSLREVLNDTISYPDGTFMDWEFKISVLYDIAKGMSYLHSSKTEVHGRLKSTNCVVDSRMVVKITDFGCNSILPPKKDLWTAPEHLRQANISQKGDVYSYGIIAQEIILRKETFYTLSCRDRNEKIFRVENSNGMKPFRPDLFLETAEEKELEVYLLVKNCWEEDPEKRPDFKKIETTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADRLNFMLLPRLVVKSLKEKGFVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFDHIVDHHDVYKVETIGDAYMVASGLPKRNGNRHAIDIAKMALEILSFMGTFELEHLPGLPIWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHVSGSTIAILKRTECQFLYEVRGETYLKGRGNETTYWLTGMKDQKFNLPTPPTVENQQRLQAEFSDMIANSLQKRQAAGIRSQKPRRVASYKKGTLEYLQLNTTDKESTYF

Sequence positions 1–1073

Gray shows the full canonical sequence. Colored spans mark the listed intervals.

Signal peptide

1–23

Membrane-spanning segments

431–454 (Helical)

Source-annotated domains

24–430 (Extracellular); 455–1073 (Cytoplasmic)

Source-annotated extracellular intervals

24–430

UniProt annotates the following extracellular intervals.

One-based positions include both interval endpoints.
Start	End	Length	Sequence and source
24	430	407	Sequence and source Basis reviewed-uniprot-topological-domain Evidence class source-annotated-topology Sequence SHA-256 caf56803329de39db904dc7c692f1accde255fc6a01332274e292ab78f198a98 Amino-acid sequence for this intervalSQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQ

Sequence and topology sources

Source role: canonical-sequence
Source: alphafold-db-canonical-uniprot-field
Extracted field: uniprotSequence
Source row accession: Not recorded

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Open sequence source

Source role: reviewed-topology
Source: UniProt reviewed human surface-field snapshot
Extracted field: Not recorded
Source row accession: P25092

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subcellular_location: SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23269669}; Single-pass type I membrane protein {ECO:0000269|PubMed:23269669}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:23269669}; Single-pass type I membrane protein {ECO:0000269|PubMed:23269669}. Note=The 145 kDa plasma membrane form of GUCY2C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form. {ECO:0000269|PubMed:23269669}.
transmembrane: TRANSMEM 431..454; /note="Helical"; /evidence="ECO:0000255"
topological_domain: TOPO_DOM 24..430; /note="Extracellular"; /evidence="ECO:0000255"; TOPO_DOM 455..1073; /note="Cytoplasmic"; /evidence="ECO:0000255"
signal_peptide: SIGNAL 1..23; /evidence="ECO:0000255"
lipidation: Not recorded

Download all canonical target sequences (FASTA)

Protein identity and location

Protein: Guanylyl cyclase C
Protein location: Transmembrane protein
Canonical sequence: P25092 · 1,073 amino acids
Extracellular region: UniProt annotates extracellular residues 24–430.

More structures and generated models

Structure snapshots

Expression in normal tissues

RNA specificity: Tissue enriched
RNA distribution: Detected in some
RNA tissue-specific nTPM: intestine: 64.2
Protein specificity: Tissue enriched
Protein distribution: Detected in single
Immunohistochemistry reliability: Uncertain
Immunofluorescence reliability: Uncertain
Protein tissue-specific intensity: intestine: 2,256,672.6
Tissue cell-type enrichment: Colon - Colon enterocytes
Subcellular location: Vesicles

Human Protein Atlas: GUCY2C

Structures

Structures records linked to GUCY2C.
Name	Stable ID	Action	Format	Status	Claim
8GHP	S-RCSB-8GHP	Not recorded	Not recorded	not recorded	verified deposited experimental structure

Sources and downloads

UniProt protein record Target measurements (JSON)Sequence annotations (JSON)Target sequences (FASTA)

GUCY2C · ENSG00000070019 · P25092